Characterization of the Biosynthetic Gene Clusters Located on the Streptomyces Linear Plasmid
نویسندگان
چکیده
منابع مشابه
on the effect of linear & non-linear texts on students comprehension and recalling
چکیده ندارد.
15 صفحه اولRecombinational cloning of the antibiotic biosynthetic gene clusters in linear plasmid SCP1 of Streptomyces coelicolor A3(2).
The model organism Streptomyces coelicolor A3(2) harbors a 356-kb linear plasmid, SCP1. We report here development of a recombinational cloning method for deleting large segment from one telomere of SCP1 followed by replacing with the telomere of pSLA2 and sequentially inserting with the overlapping cosmids in vivo. The procedure depends on homologous recombination coupled with cleavage at telo...
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ژورنال
عنوان ژورنال: Actinomycetologica
سال: 2010
ISSN: 1881-6371,0914-5818
DOI: 10.3209/saj.saj240103